bacteria e coli atcc 25 922  (ATCC)

Journal: Scientific Reports

Article Title: Characterization, in-vitro biological and antimicrobial testing of replacing Sr/Ca in wollastonite (Ca 1 − x Sr x SiO 3 ) glass-ceramics

doi: 10.1038/s41598-026-36649-1

Figure Lengend Snippet: Negative antibacterial effects of glass and glass-ceramic samples against the Gram-negative Bacteria E. coli ATCC 25,922 ( A , B ) respectively, the Gram-positive Bacteria S. aureus ATCC 6538 ( C , D ) respectively. 100 mg of each sample placed in ~ 9 mm diameter wells corresponding to Sr²⁺ concentrations of 0.125, 0.25, and 0.50 mg/mL for W1Sr, W2Sr, and W3Sr, respectively.

Article Snippet: Fig. 13 Negative antibacterial effects of glass and glass-ceramic samples against the Gram-negative Bacteria E. coli ATCC 25,922 ( A , B ) respectively, the Gram-positive Bacteria S. aureus ATCC 6538 ( C , D ) respectively.

Techniques: Bacteria

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: The expression of IL-8 and pBD2 after cells were challenged with E. coli. IPEC-J2 cells were challenged with E. coli for 1 h when cells grew to 80% confluence, then incubated for an additional 0 h, 2 h, 4 h, and 8 h respectively. ( a ) The relative expression of IL-8. ( b ) The relative expression of pBD2. Data are the mean ± SD. * indicated that it was significantly difference compared with that in the control group ( P < 0.05)

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Incubation, Control

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: pBD2 effected on the expression of IL-8 and endogenous pBD2 after cells were challenged with E. coli. IPEC-J2 cells were incubated with different concentrations of pBD2 (0, 20, 40, 80 µg/mL) for an additional 2 h, 4 h and 8 h respectively, after being challenged with E. coli for 1 h. ( a ) The relative expression of IL-8. ( b ) The relative expression of pBD2. Data are the mean ± SD. * indicated that it had a significant difference compared with that without pBD2 ( P < 0.05)

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Incubation

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: The expression of TLRs after cells were challenged with E. coli. IPEC-J2 cells were challenged with E. coli for 1 h when cells grew to 80% confluence, then incubated for an additional 0 h, 2 h, 4 h, and 8 h respectively. Data are the mean ± SD. * indicated that it had a significant difference compared with that in the control group ( P < 0.05)

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Incubation, Control

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: pBD2 effected on the expression of TLRs after cells were challenged with E. coli. IPEC-J2 cells were incubated with different concentrations of pBD2 (0, 20, 40, 80 µg/mL) for an additional 2 h ( a ), 4 h ( b ) and 8 h ( c ) after IPEC-J2 cells were challenged with E. coli for 1 h, respectively. Data are the mean ± SD. * indicated that it had a significant difference compared with that without pBD2 (P < 0.05)

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Incubation

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: The expression of key genes in TLRs-NF-κB/MAPK signaling pathway after cells were challenged with E. coli. IPEC-J2 cells were challenged with E. coli for 1 h when cells grew to 80% confluence, then incubated for an additional 0 h, 2 h, 4 h, and 8 h respectively. Data are the mean ± SD. * indicated that it had a significant difference compared with that in the control group ( P < 0.05)

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Incubation, Control

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: pBD2 effected on the expression of key genes in TLRs-NF-κB/MAPK signaling pathway after cells were challenged with E. coli. IPEC-J2 cells were incubated with different concentrations of pBD2 (0, 20, 40, 80 µg/mL) for an additional 2 h ( a ), 4 h ( b ) and 8 h ( c ) after IPEC-J2 cells were challenged with E. coli for 1 h, respectively. Data are the mean ± SD. * indicated that it had a significant difference compared with that without pBD2 ( P < 0.05)

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Incubation

Journal: BMC Veterinary Research

Article Title: Porcine beta defensin 2 attenuates inflammatory responses in IPEC-J2 cells against Escherichia coli via TLRs-NF-κB/MAPK signaling pathway

doi: 10.1186/s12917-024-04220-7

Figure Lengend Snippet: pBD2 effected on the expression of p-p65, p-p38 and p-JNK after cells were challenged with E. coli. The protein expression of p-p65 ( a ), p-p38 ( b ) and p-JNK ( c ) were determined by western blot. 1 indicated the control. 2–5 indicated that IPEC-J2 cells were incubated with different concentrations of pBD2 (0, 20, 40, 80 µg/mL) for 2 h after cells were challenged with E. coli for 1 h, respectively

Article Snippet: The cells were challenged with E. coli ATCC 25,922 (20 μl bacteria of 1×10 9 CFU/mL was added, MOI ≈ 100) for 1 h when the cells grew to about 80% confluence in the 6-well plate, and the cultural solution was removed and washed with PBS for three times, then cells were collected after incubated for an additional 2 h, 4 h and 8 h respectively.

Techniques: Expressing, Western Blot, Control, Incubation

Journal: Molecules

Article Title: Liquid and Vapour Phase of Lavandin ( Lavandula × intermedia ) Essential Oil: Chemical Composition and Antimicrobial Activity

doi: 10.3390/molecules24152701

Figure Lengend Snippet: Growth inhibition halo (mm) for LEO and the positive control (gentamicin) against the five tested Gram + and Gram − bacterial strains ( p values ≤ 0.05).

Article Snippet: Five microbial cultures belonging to gram-positive (G + ) and gram-negative (G − ) bacteria were tested: E. coli American Type Culture Collection-ATCC 25,922 (G − ), B. cereus ATCC 10,876 (G + ), A. bohemicus DSM 102,855 (G − ), K. marina DSM 16,420 (G + ), and P. fluorescens ATCC 13,525 (G − ).

Techniques: Inhibition, Positive Control, Diffusion-based Assay

Journal: Molecules

Article Title: Liquid and Vapour Phase of Lavandin ( Lavandula × intermedia ) Essential Oil: Chemical Composition and Antimicrobial Activity

doi: 10.3390/molecules24152701

Figure Lengend Snippet: Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) means for LEO expressed in percentage values (%) and the positive control (gentamicin) expressed in µg/mL against the five tested bacterial strains.

Article Snippet: Five microbial cultures belonging to gram-positive (G + ) and gram-negative (G − ) bacteria were tested: E. coli American Type Culture Collection-ATCC 25,922 (G − ), B. cereus ATCC 10,876 (G + ), A. bohemicus DSM 102,855 (G − ), K. marina DSM 16,420 (G + ), and P. fluorescens ATCC 13,525 (G − ).

Techniques: Concentration Assay, Positive Control

Journal: Molecules

Article Title: Liquid and Vapour Phase of Lavandin ( Lavandula × intermedia ) Essential Oil: Chemical Composition and Antimicrobial Activity

doi: 10.3390/molecules24152701

Figure Lengend Snippet: LEO vapour phase test showing minimum inhibitory concentration (MIC) and minimum bactericidal concentration means (MBC) expressed in µL against the five bacteria tested.

Article Snippet: Five microbial cultures belonging to gram-positive (G + ) and gram-negative (G − ) bacteria were tested: E. coli American Type Culture Collection-ATCC 25,922 (G − ), B. cereus ATCC 10,876 (G + ), A. bohemicus DSM 102,855 (G − ), K. marina DSM 16,420 (G + ), and P. fluorescens ATCC 13,525 (G − ).

Techniques: Concentration Assay, Bacteria